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Journal: Oxidative Medicine and Cellular Longevity
Article Title: Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in A β -Transgenic Caenorhabditis elegans
doi: 10.1155/2021/9966223
Figure Lengend Snippet: The effects of palmatine on sod-3p ::GFP expression, SOD, and CAT activities. (a, b) The images of sod-3p ::GFP treated with or without palmatine (0.2 mM). (c) Quantification of sod-3p ::GFP fluorescence intensity. The transgenic CF1553 strain was cultured at 20°C for two days on NGM plates with or without palmatine (0.2 mM). The images were obtained by using a Mshot MF52 inverted fluorescence microscope. Quantified sod-3p ::GFP intensity was done by ImageJ software. The result was displayed as mean ± SD ( n = 20–25), and the statistical analysis was carried out with unpaired t -test. ∗∗∗ p < 0.001. (d) The effect of palmatine on the enzyme activity of SOD in wild-type worms. (e) The effect of palmatine on the enzyme activity of CAT in wild-type worms. W ild-type worm s were cultured from L1- to L4-stage at 20°C on NGM plates with or without palmatine (0.2 mM), and then, the worms were lysed to detect the enzyme activities of SOD and CAT. The result was displayed as mean ± SD, and the statistical analysis was done by unpaired t -test. ∗ p < 0.05; ∗∗ p < 0.01.
Article Snippet: The detection assay kits of
Techniques: Expressing, Fluorescence, Transgenic Assay, Cell Culture, Microscopy, Software, Activity Assay